The new emerging stems and buds of pop lar 110 ( Populus deltoids ×P. m ax im ow iczii cv‘Eridano’)were used as exp lants and incubated onMSmedia supp lemented with various hormone combinations. Once the successive and rooting p lants were obtained through selection of culturalmedium formula, leaves and internodals wereexcised from tubed-culture p lanlets and induced to form regenerated p lants. The result showedMS + 62BA 0. 5 mg·L-1 +NAA 0. 1 mg·L-1 + TDZ 0. 02 mg·L-1 was beneficial for leaves to inducing adventitious buds; The propermedium for using internodals as exp lantswasMS + 6-BA 0. 3 mg·L-1 +NAA 0. 05 mg·L-1 + TDZ 0. 02 mg·L-1 ;while the medium ofMS + 62BA 0. 3 mg·L-1 +NAA 0. 1 mg·L-1 was p roper to culturing the stronger tubedp lanlets. The op timum medium for p roducing rootswas 1 /2MS + IBA 0. 5 mg·L-1. The effect of different incisingmode and growth state of leaves and internodals on inducing adventitiouswas discussed. In vitro vegetative p ropagation and p lant rootingwere op timized to establish the high2efficient tissue culture rep roduction system of pop lar 110,which make it possible for tree breeding and genetic transformation of this species.